Characterization of polysaccharides from Ganoderma spp. using saccharide mapping
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- 作者：Ding-Tao Wu ; Jing Xie ; De-Jun Hu ; Jing Zhao ; Shao-Ping Li
- 关键词：ANTS ; 8-aminonaphthalene-1 ; 3 ; 6-trisulfonic acid ; DP ; degree of polymerization ; DMSO ; dimethyl sulfoxide ; ELSD ; evaporative light scattering detector ; HPTLC ; high-performance thin-layer chromatography ; HPSEC ; high-performance size-exclusion chromatography
- 刊名：Carbohydrate Polymers
- 出版年：2013
- 出版时间：12 September, 2013
- 年：2013
- 卷：97
- 期：2
- 页码：398-405
- 全文大小：2737 K
文摘
Polysaccharides from Ganoderma spp. and their adulterants were firstly investigated and compared using saccharide mapping, enzymatic (endo-1,3-¦Â-d-glucanase and pectinase) digestion followed by polysaccharide analysis using carbohydrate gel electrophoresis analysis. The results showed that both 1,3-¦Â-d-glucosidic and 1,4-¦Á-d-galactosiduronic linkages were existed in Lingzhi (Ganoderma lucidum and Ganoderma sinense), and the similarity of polysaccharides from G. lucidum and G. sinense was high, which may contribute to rational use of Lingzhi. Different species of Ganoderma and their adulterants can be differentiated based on the saccharide mapping, which is helpful to well understand the structural characters of polysaccharides from different species of Ganoderma and to improve the quality control of polysaccharides in Lingzhi.