Amplified detection of saccharide based on redox-poly(phenol-co-3-hydroxyphenylboronic acid) coupling with a redox cycling

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• 作者：Jian Li ; ^{jli@nwu.edu.cn" class="auth_mail}Author Vitae ; Linlin LiuAuthor Vitae ; Pengge WangAuthor Vitae ; Ying YangAuthor Vitae ; Jianbin ZhengAuthor Vitae
• 关键词：Electrochemical sensor ; Redox-polymer ; Redox cycling ; Phenylboronic acid ; Saccharide
• 刊名：Sensors and Actuators B: Chemical
• 出版年：31 July 2014
• 年：2014
• 卷：198
• 期：Complete
• 页码：219-224
• 全文大小：1081 K

文摘

Phenol and 3-hydroxyphenylboronic acid were electrochemically co-polymerized on a glassy carbon electrode in the presence of phenazine methosulfate (PMS), to form redox-poly(phenol-co-3-hydroxyphenylboronic acid). The as-synthesized phenylboronic acid bearing redox-polymer was characterized by UV–visible spectroscopy and cyclic voltammetry. The behavior of Fe(CN)₆³⁻ catalytic reduction by electro-generated PMS_red via a redox cycling was studied. With the addition of saccharide, a stable negative charged surface was formed owing to the generation of phenylboronate ester and then limited the access of Fe(CN)₆³⁻ due to electrostatic repulsion. The electrochemical signal amplification of attenuated Fe(CN)₆³⁻ was dependent on the concentration of saccharide. The dynamic ranges of the fabricated electrochemical sensor were 1–100 渭M for d-fructose, 10–300 渭M for d-mannose and 20–500 渭M for d-glucose, respectively. The detection limits were ca. 0.3 渭M for d-fructose, ca. 3.5 渭M for d-mannose and ca. 7.8 渭M for d-glucose, respectively.