Synthesis, characterization and optimization of a two-step immobilized lipase

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• 作者：Kang Zhao^a ; ^b ; Xi Cao^a ; Qinjian Di^a ; Meng Wang^a ; Hao Cao^a ; Li Deng^a ; ^{dengli@mail.buct.edu.cn} ; Junfeng Liu^a ; Fang Wang^a ; Tianwei Tan^a
• 关键词：Immobilization ; Lipase ; SEM ; Mechanisms ; Biocatalyst
• 刊名：Renewable Energy
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：103
• 期：Complete
• 页码：383-387
• 全文大小：1525 K
• 卷排序：103

文摘

Immobilized lipase could be reused 11 times while the biodiesel yield remained in excess of 70%. A two step lipase immobilization was developed to convert waste cooking oil to biodiesel. Underlying mechanisms associated with the two-step immobilization processes were established. Formation mechanism and internal structure of the immobilized lipase was explored. Gelatine had a positive effect on the enzyme activity and immobilized stability.