A rupture detection algorithm for the DNA translocation detection though biological nanopore

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• 作者：T. Osaki^a ; J.P. Barbot^b ; R. Kawano^a ; H. Sasaki^a ; O. Franç ; ais^b ; B. Le Pioufle^b ; S. Takeuchi^a
• 关键词：Type your keywords here ; Separated by semicolons ; DNA translocation ; Signal processing ; Nanopore
• 刊名：Procedia Engineering
• 出版年：2010
• 出版时间：2010
• 年：2010
• 卷：5
• 期：Complete
• 页码：796-799
• 全文大小：904 K

文摘

We propose in this paper a new methodology for the treatment of electrical signal of DNA translocation through α-hemolysin biological nanopore formed on an artificial lipidic membrane reconstituted on a microfluidic 96 wells microarray. The signal treatment algorithm is capable to detect translocation events through the pore, within very long and noisy recordings on multiple chambers of our microarray. The firing events are precisely detected, as well as the duration and amplitude of the event, thanks to a rupture detection algorithm. This signal treatment analysis, in association to our artificial bilayer microarray technology, might bring a substantial step beyond highly parallelized biosensing of DNA on a chip.