In聽vitro cytotoxicity studies of palladacyclic complexes containing the symmetric diphosphine bridging ligand. Studies of their interactions with DNA and BSA

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• 作者：Kazem Karami ; Mahboubeh Hosseini-Kharat ; Hojjat Sadeghi-Aliabadi ; Janusz Lipkowski ; Mina Mirian
• 关键词：Palladacyclic complex ; FS-DNA binding ; BSA binding ; Cytotoxicity ; Crystal structure
• 刊名：European Journal of Medicinal Chemistry
• 出版年：12 February, 2014
• 年：2014
• 卷：73
• 期：Complete
• 页码：8-17
• 全文大小：1747 K

文摘

The reactions between [Pd<sub>2sub>{(C,N)-C<sub>6sub>H<sub>4sub>CH<sub>2sub>NH(Et)}<sub>2sub>(渭-X)<sub>2sub>] (X聽=聽Cl or Br) and 1,2-bis(diphenylphosphino)ethane (dppe) in the 1:1 molar ratio resulted in the dppe-bridged Pd(II) complexes, [Pd<sub>2sub>{(C,N)-C<sub>6sub>H<sub>4sub>CH<sub>2sub>NH(Et)}<sub>2sub>(渭-dppe)(Cl)<sub>2sub>] (<strong class="boldFont">1strong>) and [Pd<sub>2sub>{(C,N)-C<sub>6sub>H<sub>4sub>CH<sub>2sub>NH(Et)}<sub>2sub>(渭-dppe)(Br)<sub>2sub>] (<strong class="boldFont">2strong>), respectively, which were characterized by elemental analyses, infrared (IR), <sup>1sup>H- and <sup>31sup>P{<sup>1sup>H} NMR spectroscopy. The molecular structure of <strong class="boldFont">1strong> was determined by single-crystal X-ray diffraction. In聽vitro cytotoxicity of <strong class="boldFont">1strong>, <strong class="boldFont">2strong>, dppe, PhCH<sub>2sub>NH(Et) and cisplatin were carried out against four human tumor cell lines. The interactions of complexes towards DNA and protein are investigated. The results suggested that both complexes could interact with FS-DNA through the intercalation mode. Moreover, the reactivity towards BSA revealed that the microenvironment and the secondary structure of BSA were changed in the presence of Pd(II) complexes.