Multiplex and accurate quantification of acute kidney injury biomarker candidates in urine using Protein Standard Absolute Quantification (PSAQ) and targeted proteomics

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• 作者：Benoî ; t Gilquin^a ; ^b ; ^c ; Mathilde Louwagie^a ; ^b ; ^c ; Michel Jaquinod^a ; ^b ; ^c ; Alexandre Cez^d ; Guillaume Picard^a ; ^b ; ^c ; Leila El Kholy^a ; ^b ; ^c ; Brigitte Surin^e ; Jé ; rô ; me Garin^a ; ^b ; ^c ; Myriam Ferro^a ; ^b ; ^c ; Thomas Kofman^f ; Caroline Barau^g ; Emmanuelle Plaisier^d ; ^e ; ^h ; Pierre Ronco^d ; ^e ; ^h ; Virginie Brun^a ; ^b ; ^c ; ^{virginie.brun@cea.fr}
• 关键词：AKI ; acute kidney injury ; L-FABP ; liver fatty acid-binding protein ; MED-FASP ; multiple enzyme digestion &ndash ; filter aided sample preparation ; MIOX ; myo-inositol oxygenase ; NGAL ; Neutrophil gelatinase-associated lipocalin ; PCK1 ; phosphoenolpyruvate carboxykinase 1 ; SRM ; Selected Reaction Monitoring ; PSAQ ; Protein Standard Absolute Quantification
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：164
• 期：Complete
• 页码：77-84
• 全文大小：813 K
• 卷排序：164

文摘

There is a need for multiplex and quantitative methods to speed-up the development of acute kidney injury biomarkers. We report the development of a targeted proteomic assay to quantify 4 AKI biomarker candidates in urine samples. Thanks to the use of isotopically-labeled proteins as quantification standards, the assay showed excellent performance. The assay confirmed the relevance of NGAL and L-FABP in AKI diagnosis.