Enhanced production of insect raw-starch-digesting alpha-amylase accompanied by high erythritol synthesis in recombinant Yarrowia lipolytica fed-batch cultures at high-cell-densities
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- 作者：Ewelina Celińska ; celinska@up.poznan.pl ; Monika Borkowska ; Wojciech Białas
- 关键词：Amylase ; Fed-batch cultures ; Heterologous over-expression ; High-cell-density cultures ; Yarrowia lipolytica ; Sitophilus oryzae
- 刊名：Process Biochemistry
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：52
- 期：Complete
- 页码：78-85
- 全文大小：612 K
- 卷排序：52
文摘
Raw-starch digesting α-amylase from rice weevil was produced in Y. lipolytica host. Intermittent fed-batch cultures at high-cell-densities were conducted. The peak activity of the enzyme was improved over 160-fold. High erythritol and moderate citric acid titers accompanied the process. Carbon and nitrogen fluxes distribution during the process was calculated.