Engineering Yarrowia lipolytica for arachidonic acid production through rapid assembly of metabolic pathway

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• 作者：Hu-Hu Liu^a ; Catherine Madzak^b ; Mei-Li Sun^a ; Lu-Jing Ren^a ; ^e ; Ping Song^a ; ^e ; He Huang^c ; ^d ; ^e ; Xiao-Jun Ji^a ; ^e ; ^{xiaojunji@njtech.edu.cn}
• 关键词：Yarrowia lipolytica ; Arachidonic acid ; In vivo ; Pathway assembly ; Synthetic biology
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 March 2017
• 年：2017
• 卷：119
• 期：Complete
• 页码：52-58
• 全文大小：945 K
• 卷排序：119

文摘

A in vivo one-step pathway assembly was used for ARA biosynthesis in Y. lipolytica. The assembly efficiency was affected by the length of overlapping region. The assembled pathway performed long-term genetic stability in Y. lipolytica. The codon-optimized genes from M. alpina could function in Y. lipolytica.