Engineering Yarrowia lipolytica for efficient γ-linolenic acid production

详细信息    查看全文

• 作者：Mei-Li Sun^a ; Catherine Madzak^b ; Hu-Hu Liu^a ; Ping Song^a ; ^e ; Lu-Jing Ren^a ; ^e ; He Huang^c ; ^d ; ^e ; Xiao-Jun Ji^a ; ^e ; ^{xiaojunji@njtech.edu.cn}
• 关键词：γ-Linolenic acid ; Yarrowia lipolytica ; △6-Desaturase ; Metabolic engineering ; Temperature-shift strategy
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：117
• 期：part_PA
• 页码：172-180
• 全文大小：1700 K
• 卷排序：117

文摘

A recombinant Y. lipolytica for GLA production was constructed. The effect of temperature on production of GLA by the recombinant strain was investigated. DCW, lipid and GLA production were enhanced by temperature shift strategies. The best culture condition was 28 °C followed by 6-day culture at 20 °C.