Molecular cloning and characterization of LrTLR4, analysis of its inductive expression and associated down-stream signaling molecules following lipopolysaccharide stimulation and Gram-negative bacterial infection
文摘
The cDNA of Labeo rohita TLR4 (LrTLR4) consisted of 2729 bp, encoding 823 aa. LrTLR4 was phylogenetically related to common carp TLR4bb and zebrafish TLR4b. LPS-treatment induced LrTLR4, MyD88, TRIF, TRAF6, IL-6, IL-8 and type-I IFN gene expression. In A.hydrophila infection, LrTLR4 gene expression was significantly induced.