This research paper presents a novel system for detecting the effects of bacteriophages on human gut.

The system studied the effects of bacteriophages that infect Escherichia coli O157:H7, E. coli O104:H4 and Salmonella typhimurium on human gut.

Enterobacteria phage 933W, Enterobacteria phage VT2-Sa and Enterobacteria phage P22 which are bacteriophages that infect E. coli O157:H7, E. coli O104:H4 and S. typhimurium, were chose to study its effect on seven bacterium families exist in human gut.

Three interactions, the bacteriophages proteins and the human gut bacterium strains proteins were detected.

From the resulted interactions, several effects were detected such as: antibacterial activity against a number of bacterial species in human gut, regulation of cellular differentiation and organogenesis during gut, lung, and heart development, ammonia assimilation in bacteria, yeasts, and plants, energizing defense system and its function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in human gut.