Grape seed and skin extract reduces pancreas lipotoxicity, oxidative stress and inflammation in high fat diet fed rats

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• 作者：Faten Aloui^a ; ^b ; Kamel Charradi^a ; ^b ; ^{charradi3@yahoo.com} ; Aziz Hichami^c ; Selvakumar Subramaniam^c ; Naim Akhtar Khan^c ; Ferid Limam^b ; Ezzedine Aouani^a ; ^b
• 关键词：Obesity ; Pancreas ; Grape seed and skin extract ; Oxidative stress ; Inflammation
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：84
• 期：Complete
• 页码：2020-2028
• 全文大小：2413 K
• 卷排序：84

文摘

Obesity is related to an elevated risk of diabetes and the mechanisms whereby fat adversely affects the pancreas are poorly understood. We studied the effect of a high fat diet (HFD) on pancreas steatosis, oxidative stress and inflammation as well as the putative protection afforded by grape seed and skin extract (GSSE). HFD induced body weight gain, without affecting insulinemia, nor glycemia and dropped adiponectemia. HFD also provoked the ectopic deposition of cholesterol and triglyceride, and an oxidative stress characterized by increased lipoperoxidation and carbonylation, inhibition of antioxidant enzyme activities such as CAT, GPx and SOD, depletion of zinc and a concomitant increase in calcium and H2O2. HFD induced pro-inflammatory chemokines mRNA as RANTES and MCP1 as well as cytokines expression as TNFα, IL6 and IL1β. Importantly GSSE counteracted all the deleterious effects of HFD on pancreas in vivo i-e lipotoxicity, oxidative stress and inflammation.In conclusion, GSSE could find potential applications in fat-induced pancreas lipotoxicity and dysfunction.