Lipase-catalyzed synthesis of capsiate analog using vanillyl alcohol and conjugated linoleic acid under vacuum system

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• 作者：Seung In Hong^a ; ^b ; ¹ ; Na Ma^a ; ¹ ; Inhwan Kim^a ; Jungju Seo^c ; In-Hwan Kim^a ; ^{k610in@korea.ac.kr}
• 关键词：Lipozyme RM IM ; Conjugated linoleic acid (CLA) ; Esterification ; Capsiate analog ; Vacuum
• 刊名：Process Biochemistry
• 出版年：2012
• 出版时间：December, 2012
• 年：2012
• 卷：47
• 期：12
• 页码：2317-2322
• 全文大小：567 K

文摘

This study focused on the application of vacuum system to synthesize capsiate analogs. The capsiate analogs containing conjugated linoleic acid (CLA) was successfully synthesized in solvent free system via lipase-catalyzed esterification. This esterification was carried out using vanillyl alcohol and CLA as substrates, and Lipozyme RM IM from Rhizomucor miehei as a biocatalyst. The best reaction condition was a molar ratio of 1:2 (vanillyl alcohol to CLA), a reaction temperature of 50 ¡ãC, and a lipase loading of 10 % (w/w, based on total substrates). Application of vacuum increased the yield of capsiate analog as well as the reaction rate. When the vacuum levels were between 66.7 kPa and 1.3 kPa, an equilibrium yield of 100 mol % was achieved. The maximum yield was approached after only 3 h of reaction at the vacuum levels of higher than 13.3 kPa. The content of 9c,11t-CLA in capsiate analog synthesized was higher than that of 10t,12c-CLA.