Performance of two immobilized lipases for interesterification between canola oil and fully-hydrogenated canola oil under supercritical carbon dioxide

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• 作者：Ehsan Jenab ; Feral Temelli ; ^{feral.temelli@ualberta.ca" class="auth_mail} ; Jonathan M. Curtis ; Yuan-Yuan Zhao
• 关键词：Canola oil ; Enzymatic interesterification ; Fully-hydrogenated canola oil ; Immobilized lipase ; Supercritical carbon dioxide
• 刊名：Lebensmittel-Wissenschaft und -Technologie
• 出版年：September, 2014
• 年：2014
• 卷：58
• 期：1
• 页码：263-271
• 全文大小：1410 K

文摘

The performance and stability of Lipozyme RM IM and TL IM for the interesterification between canola oil and fully-hydrogenated canola oil (FHCO) in the presence of SCCO₂ were studied using a high pressure batch stirred reactor at 65聽掳C/17.5聽MPa. The influence of exposure time (4, 8, and 12聽h) in SCCO₂ and pressurization/depressurization cycles of up to 12 times on the enzyme's resultant activity were studied. There was no significant difference (p聽>聽0.05) in the performance of the two enzymes over reaction time, reaching a constant degree of interesterification of about 23% after 2聽h. Although FE-SEM images illustrated some morphological changes on the surface of the enzymes after 4 cycles of 7聽h each, similar degrees of interesterification were achieved after each cycle. However, the amount of reaction intermediates decreased by 50-60% in the product obtained by using SCCO₂-treated enzymes after 12 pressurization/depressurization cycles compared to untreated enzymes, while there were no significant changes in the conformational and morphological structure of the treated enzymes based on FTIR and FE-SEM analysis. Findings enhance our understanding of enzymatic conversions of lipids under high pressure CO₂, targeting production of base-stock for zero-trans margarines.