Locked nucleic acids biosensor for detection of BCR/ABL fusion gene using benzoate binuclear copper (II) complex as hybridization indicator

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• 作者：Liqing Lin^a ; Jie Kang^a ; Shaohuang Weng^a ; Jinghua Chen^a ; Ailin Liu^a ; Xinhua Lin^a ; ^{xinhua63@163.com"" rel=""nofollow} ; Yuanzhong Chen^b ; ^{chenyz@pub3.fz.fj.cn"" rel=""nofollow} [Author vitae]
• 关键词：CuR₂ ; DNA hybridization ; BCR/ABL fusion gene ; Locked nucleic acids ; Electrochemical biosensor
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2011
• 出版时间：5 July 2011
• 年：2011
• 卷：155
• 期：1
• 页码：1-7
• 全文大小：834 K

文摘

Benzoate binuclear copper (II) complex, [Cu₂(C₇H₅O₂)₄(C₂H₆O)₂] (abbreviated as CuR₂) was prepared and its interaction with double-stranded salmon sperm DNA (dsDNA) in pH 7.4 phosphate buffer solution was studied by electrochemical experiments at the Au electrode (AuE). It was revealed that CuR₂ presented an excellent electrochemical activity on AuE and could bind with dsDNA by intercalation mode. The CuR₂ was further utilized as a new indicator in the fabrication of an electrochemical DNA biosensor for detection of BCR/ABL fusion gene. The biosensor based on nanogold (NG) modified AuE was developed by using thiolated-hairpin locked nucleic acids (LNA) as the capture probe for hybridization with BCR/ABL fusion gene. The results indicated this new method has excellent specificity for single-base mismatch and complementary after hybridization. The constructed electrochemical DNA biosensor achieved a detection limit of 1.0 × 10⁻¹⁰ M for complementary target DNA with a good stability.