Prevention mechanisms of glucose intolerance and obesity by cacao liquor procyanidin extract in high-fat diet-fed C57BL/6 mice

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• 作者：Yoko Yamashita ; Masaaki Okabe ; Midori Natsume ; Hitoshi Ashida
• 关键词：AMP-activated protein kinase ¦Á ; Cacao liquor procyanidin ; Glucose transporter 4 ; Hyperglycemia ; Obesity ; Uncoupling protein
• 刊名：Archives of Biochemistry and Biophysics
• 出版年：2012
• 出版时间：15 November, 2012
• 年：2012
• 卷：527
• 期：2
• 页码：95-104
• 全文大小：643 K

文摘

In this study, we investigated whether cacao liquor procyanidin (CLPr) extract, which consists of 4.3 % catechin, 6.1 % epicatechin, 39.4 % procyanidins and others, ameliorated hyperglycemia and obesity in C57BL/6 mice fed a control or high-fat diet for 13 weeks. CLPr suppressed high-fat diet-induced hyperglycemia, glucose intolerance and fat accumulation in white adipose tissue. CLPr also promoted translocation of glucose transporter 4 (GLUT4) and phosphorylation of AMP-activated protein kinase ¦Á (AMPK¦Á) in the plasma membrane of skeletal muscle and brown adipose tissue. Phosphorylation of AMPK¦Á was also enhanced in the liver and white adipose tissue. CLPr up-regulated the gene and protein expression levels of uncoupling protein (UCP)-1 in brown adipose tissue and UCP-3 in skeletal muscle. These results indicate that CLPr is a beneficial food material for the prevention of hyperglycemia and obesity. Activation of AMPK¦Á, translocation of GLUT4 and up-regulation of UCP expression in skeletal muscle and adipose tissue are involved in the molecular mechanisms by which CLPr prevents hyperglycemia and obesity.