Oil mill wastewater (OMW) was evaluated as an inductor of rhamnolipid production.
A culture medium containing corn steep liquor, molasses and OMW was designed.
Pseudomonas aeruginosa #112 produced 5.1 g rhamnolipid/l using that medium.
The critical micelle concentration of the purified rhamnolipids was 13 mg/l.
This medium represents a sustainable alternative for rhamnolipid production.