Simultaneous detection of pathogenic Listeria including atypical Listeria innocua in vegetables by a quadruplex PCR method
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- 作者：Aurelius Albert Rosimina ; 1 ; Min-Ju Kima ; 1 ; In-Sun Joob ; Soo-Hwan Suhb ; Keun-Sung Kima ; keunsung@cau.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Quadruplex PCR ; Pathogenic Listeria ; Listeria monocytogenes ; Listeria ivanovii ; Atypical Listeria innocua
- 刊名：LWT - Food Science and Technology
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：69
- 期：Complete
- 页码：601-607
- 全文大小：545 K
文摘
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A quadruplex PCR method was developed to simultaneously detect pathogenic Listeria.
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L. monocytogenes, L. ivanovii, and typical and atypical L. innocua were targeted.
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There were no cross-reactions of PCR between Listeria species or other bacteria.
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The quadruplex PCR method could detect up to 0.1 ng/μL of DNA.
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The assay could detect 1–10 CFU in 20 g vegetable samples after 24 h enrichment.