Sheet of osteoblastic cells combined with platelet-rich fibrin improves the formation of bone in critical-size calvarial defects in rabbits

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• 作者：Zhifa Wang^a ; ¹ ; Hanqing Hu^b ; ¹ ; Zhijin Li^a ; Yanming Weng^c ; Taiqiang Dai^a ; Chunlin Zong^a ; Yanpu Liu^a ; ^{liuyanpu@fmmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Bin Liu^d ; ^{kqomsliu@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Mesenchymal stem cells ; Cell sheet ; Platelet-rich fibrin ; Bone regeneration ; Rabbit calvarial critical-size defect
• 刊名：British Journal of Oral and Maxillofacial Surgery
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：54
• 期：3
• 页码：316-321
• 全文大小：1401 K

文摘

Techniques that use sheets of cells have been successfully used in various types of tissue regeneration, and platelet-rich fibrin (PRF) can be used as a source of growth factors to promote angiogenesis. We have investigated the effects of the combination of PRF and sheets of mesenchymal stem cells (MSC) from bone marrow on the restoration of bone in critical-size calvarial defects in rabbits to find out whether the combination promotes bony healing. Sheets of MSC and PRF were prepared from the same donor. We then implanted the combined MSC and PRF in critical-size calvarial defects in rabbits and assessed bony restoration by microcomputed tomography (microCT) and histological analysis. The results showed that PRF significantly increased bony regeneration at 8 weeks after implantation of sheets of MSC and PRF compared with sheets of MSC alone (p = 0.0048). Our results indicate that the combination of sheets of MSC and PRF increases bone regeneration in critical-size calvarial defects in rabbits, and provides a new way to improve skeletal healing.