NPM1-mutated acute myeloid leukemia of monocytic or myeloid origin exhibit distinct immunophenotypes

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• 作者：Yan-Rong Liu ; ^{yrliu163@163.com} ; Hong-Hu Zhu ; Guo-Rui Ruan ; Ya-Zhen Qin ; Hong-Xia Shi ; Yue-Yun Lai ; Yan Chang ; Ya-Zhe Wang ; Dan Lu ; Le Hao ; Jin-Lan Li ; Ling-Di Li ; Bin Jiang ; Xiao-Jun Huang
• 关键词：Acute myeloid leukemia ; Nucleophosmin mutation ; Immunophenotype ; Flow cytometry
• 刊名：Leukemia Research
• 出版年：2013
• 出版时间：July, 2013
• 年：2013
• 卷：37
• 期：7
• 页码：737-741
• 全文大小：405 K

文摘

Acute myeloid leukemia with mutated nucleophosmin (NPM1m+AML) is a heterogeneous entity. We investigated whether NPM1m+AML with monocytic or myeloid differentiation have distinct immunophenotype. The study included 160 NPM1m+AMLpatients and 178 AML patients without NPM1 mutation and recurrent cytogenetic abnormality (NPM1wt?AML). We analyzed the immunophenotype by flow cytometry. NPM1 mutation was detected by PCR. Compared with NPM1wt?AML patients, NPM1m+AML patients showed higher positive rates of CD33 and CD9 and lower positive rates of CD34, HLA-DR, CD7, CD15 and CD117 (all P < 0.05). HLA-DR, CD64, CD14, CD11b, CD15, CD4, CD9 and CD10 were higher (P < 0.001) and CD117 was lower (P < 0.01) in monocytic NPM1m+AML compared with myeloid NPM1m+AML. Similar rates of lymphoid antigen (CD19, CD2, and CD7) and myeloid antigen (CD13, CD33) positivity were detected in monocytic and myeloid NPM1m+AML. Compared with NPM1wt?AML, CD34 expression was lower both in myeloid and monocytic NPM1m+AML subgroups, although HLA-DR was lower in NPM1m+AML compared with NPM1wt?AML only in myeloid subgroup. Comparisons of NPM1m+AML and NPM1wt?AML showed no differences in monocyte-associated markers such as CD14 and CD11b in myeloid and monocytic subgroup. Myeloid NPM1m+AML correlated with the female gender (P = 0.001), lower WBC counts (P = 0.04) and higher WT1 transcripts (P = 0.006) compared with monocytic NPM1m+AML.These results suggested monocytic and myeloid-derived NPM1m+AML exhibit distinct immunophenotypes.