The effects of rutin and baicalin on the interaction of curcumin with HSA were studied.
The fluorescence quenching of HSA by curcumin is a combined quenching process.
Flavonoids had no influence on the combined quenching mechanism.
The binding affinity of curcumin with HSA was increased in the presence of flavonoids.
Rutin/bacalin and curcumin may simultaneously bind in different regions of site I.