Immunohistochemical localization of the calcitonin gene-related peptide binding site in the primate trigeminovascular system using functional antagonist antibodies
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- 作者：Silke Millera ; &dagger ; ; silkem@amgen.com" class="auth_mail" title="E-mail the corresponding author ; Hantao Liua ; &dagger ; ; Karin Warfvingeb ; Licheng Shia ; Mary Dovlatyana ; Cen Xua ; Lars Edvinssonb
- 关键词：BBB ; blood&ndash ; brain barrier ; BSA ; bovine serum albumin ; cAMP ; cyclic adenosine monophosphate ; CGRP ; calcitonin gene-related peptide ; CHO ; Chinese hamster ovary ; CLR ; calcitonin receptor-like receptor ; CNS ; central nervous system ; DAB ; 3 ; 3&prime ; -diaminobenzidine ; DAPI ; 4&prime ; 6-diamidino-2-phenylindole ; FBS ; fetal bovine serum ; GFAP ; glial fibrillary acidic protein ; HEK ; human embryonic kidney ; HRP ; horseradish peroxidase ; IgG ; immunoglobulin G ; MAP-2 ; microtubule-associated protein 2 ; MBP
- 刊名：Neuroscience
- 出版年：2016
- 出版时间：22 July 2016
- 年：2016
- 卷：328
- 期：Complete
- 页码：165-183
- 全文大小：9613 K
文摘
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Antibodies against the CLR/RAMP1 extracellular domains block CGRP binding and function in vitro.
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Antibodies display specificity in Western blot and immunohistochemistry applications.
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Immunohistochemistry localizes CGRP receptors at key sites in the trigeminovascular system.