Study of nisin adsorption on plasma-treated polymer surfaces for setting up materials with antibacterial properties

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• 作者：Layal Karam ; Charafeddine Jama ; Anne-Sophie Mamede ; Ahmad Fahs ; Guy Louarn ; Pascal Dhulster ; Nour-Eddine Chihib
• 关键词：Functional materials ; Surface properties ; Peptide adsorption ; Antibacterial properties ; Plasma functionalization
• 刊名：Reactive and Functional Polymers
• 出版年：2013
• 出版时间：November, 2013
• 年：2013
• 卷：73
• 期：11
• 页码：1473-1479
• 全文大小：861 K

文摘

Setting up antibacterial materials by nisin adsorption on surfaces depends mainly on the surface properties and the surface treatments allowing the modification of such properties. In order to investigate the factors affecting such adsorption, the native low density polyethylene (LDPE) was modified using Argon/Oxygen (Ar/O₂) plasma, nitrogen (N₂) plasma and plasma-induced grafting of acrylic acid (AA). The films were studied by various characterization techniques. The chemical surface modification was confirmed by X-ray photoelectron spectroscopy (XPS), the wettability of the surfaces was evaluated by contact angle measurements, the surface charge was determined by the zeta potential measurements, and the changes in surface topography and roughness were revealed by atomic force microscopy (AFM). Nisin was adsorbed on the native and the modified surfaces. The antibacterial activity, the nisin adsorbed amount, and the peptide distribution were compared for the four nisin-functionalized films. The highest antibacterial activity was recorded on the Ar/O₂ followed by AA then by N₂ treated films and the lowest activity was on the native film. The observed antibacterial activity was correlated to the type of the surface, hydrophobic and hydrophilic interactions, surface charge, surface topography, nisin adsorbed amount, and nisin distribution on the surfaces.