Determination of sphingomyelinase-D activity of Loxosceles venoms in sphingomyelin/cholesterol liposomes containing horseradish peroxidase

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• 作者：Marco Tú ; lio R. Gomes^a ; Gabriela Guimarã ; es^a ; Fré ; dé ; ric Fré ; zard^b ; Evanguedes Kalapothakis^c ; Joã ; o Carlos Minozzo^d ; Olga Meiri Chaim^e ; Silvio Sanches Veiga^e ; Sergio Costa Oliveira^a ; Carlos Chá ; vez-Oló ; rtegui^a ; ^{olortegi@icb.ufmg.br}
• 关键词：Anti-loxoscelic serum ; Horseradish peroxidase ; Liposomes ; Loxosceles venom ; Sphingomyelinase-D
• 刊名：Toxicon
• 出版年：2011
• 出版时间：15 March 2011
• 年：2011
• 卷：57
• 期：4
• 页码：574-579
• 全文大小：297 K

文摘

Based on degradation of sphingomyelin/cholesterol liposomes containing entrapped horseradish peroxidase, we evaluated the Sphingomyelinase-D (SMase-D) activity of scorpion, spider and snake venoms by monitoring spectrophotometrically the product of oxidation of HRP released. The results indicate that Loxosceles crude venoms (Loxosceles intermedia, Loxosceles laeta, Loxosceles gaucho and Loxosceles similis) displayed SMase-D activity in a concentration-dependent manner. Furthermore, this activity was blocked by the anti-loxoscelic antivenom. However, Tityus serrulatus scorpion venom, Phoneutria nigriventer spider venom and Bothrops jararaca, Crotalus durissus, Lachesis muta and Micrurus frontalis snake venoms did not show measurable SMase-D activity.