Hollow fibre-based LPME for the extraction of hallucinogenic muscimol, tryptophan and tryptamine from urine is reported for the first time.
Central composite designs and desirability functions were used during optimizations.
Stirring rate is the most important factor for the effective transfer of very polar compounds during HF-LPME.
Good values for method validation parameters were obtained.
The HF-LPME approach is a viable alternative for extraction of muscimol from urine samples.