Preparative isolation and purification of theaflavins and catechins by high-speed countercurrent chromatography

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• 作者：Kunbo Wang ; Zhonghua Liu ; Jian-an Huang ; Xinrong Dong ; Lubing Song ; Yu Pan ; Fang liu
• 关键词：High-speed countercurrent chromatography ; Theaflavins ; Black tea ; Catechins ; Green tea
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2008
• 出版时间：15 May 2008
• 年：2008
• 卷：867
• 期：2
• 页码：282-286
• 全文大小：379 K

文摘

High-speed countercurrent chromatography (HSCCC) has been applied for the separation of theaflavins and catechins. The HSCCC run was carried out with a two-phase solvent system composed of hexane–ethyl acetate–methanol–water–acetic acid (1:5:1:5:0.25, v/v) by eluting the lower aqueous phase at 2 ml/min at 700 rpm. The results indicated that pure theaflavin, theaflavins-3-gallate, theaflavins-3′-gallate and theaflavin-3,3′-digallate could be obtained from crude theaflavins sample and black tea. The structures of the isolated compounds were positively confirmed by ¹H NMR and ¹³C NMR, MS analysis, HPLC data and TLC data. Meanwhile, catechins including epigallocatechin gallate, gallocatechin gallate, epicatechin gallate and epigallocatechin were isolated from the aqueous extract of green tea by using the same solvent system. This study developed a modified method combined with enrichment theaflavins method by using HSCCC for separation of four individual theaflavins, especially for better separation of theaflavins monogallates.