Isolation of xanthone and benzophenone derivatives from Cyclopia genistoides (L.) Vent. (honeybush) and their pro-apoptotic activity on synoviocytes from patients with rheumatoid arthritis

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• 作者：Adam Kokotkiewicz ; Maria Luczkiewicz ; Justyna Pawlowska ; Piotr Luczkiewicz ; Pawel Sowinski ; Jacek Witkowski ; Ewa Bryl ; Adam Bucinski
• 关键词：AA ; adjuvant arthritis ; ADAMTS-4 ; aggrecanase-4 ; AP-1 ; activator protein 1 ; CD3 ; cluster of differentiation 3 ; CIA ; collagen-induced arthritis ; DAS28 ; Disease Activity Score of 28 joints ; DMEM ; Dulbecco's modified Eagle's medium ; IFN-¦Ã ; interferon-gamma
• 刊名：Fitoterapia
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：90
• 期：Complete
• 页码：199-208
• 全文大小：811 K

文摘

A fast and efficient method for the isolation of the m>Cm>-glucosidated xanthones mangiferin and isomangiferin from the South-African plant m>Cyclopia genistoidesm> was developed for the first time. The procedure involved extraction, liquid-liquid partitioning with ethyl acetate and subsequent precipitation of mangiferin and isomangiferin from methanol and acetonitrile-water fractions, respectively. Additionally, two benzophenone derivatives: 3-m>Cm>-m>¦Âm>-glucosides of maclurin and iriflophenone, were isolated from m>C. genistoidesm> extracts using semi-preparative HPLC. Apart from the above, the isolation procedure also yielded hesperidin and small amounts of luteolin. The structures of the compounds were determined by 1D and 2D NMR experiments and/or LC-DAD-ESI-MS. The selected m>Cyclopiam> constituents were screened for pro-apoptotic activity on TNF-¦Á-stimulated synovial cells isolated from rheumatoid arthritis patients. The strongest effect, measured as percent of apoptotic cells, was recorded for isomangiferin (75 % ), followed by iriflophenone 3-m>Cm>-m>¦Âm>-glucoside (71 % ), hesperidin (67 % ) and mangiferin (65 % ). The results are encouraging for further studies on the use of the above compounds in the treatment of rheumatoid arthritis.