cDNA of Poly p 1 allergen from P. paulista venom glands was cloned and sequenced. High levels of rPoly p 1 were produced in E. coli and purified by IMAC. rPoly p 1 was recognized by sera from P. paulista venom-sensitized patients. Levels of sIgE reactivity of allergic patients' sera to nPoly p 1 and rPoly p 1 were similar.