Biocatalytic asymmetric hydroxylation of hydrocarbons by free and immobilized Bacillus megaterium cells
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- 作者：Adam ; Waldemar ; Lukacs ; Zoltan ; Kahle ; Claudia ; Saha-Mö ; ller ; Chantu R. ; Schreier ; Peter
- 关键词：Bacillus megaterium ; Hydroxylation ; Hydrocarbons ; Immobilization ; Alginate
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2001
- 出版时间：January 22, 2001
- 年：2001
- 卷：11
- 期：4-6
- 页码：377-385
- 全文大小：921 K
文摘
Screening of soil bacteria with allylbenzene resulted in a Bacillus megaterium strain, which hydroxylates simple hydrocarbons in high enantiomeric excess (ee up to 99 % ). Benzylic and nonbenzylic hydroxylation products were obtained, without the usually observed high preference for the benzylic position. The immobilization of the B. megaterium cells in alginate gel effectively improved the stability of the cells and increased the amounts of products formed, without loss of enantioselectivity. The product ratio (α vs. β hydroxylation) was shifted towards benzylic hydroxylation, which suggests that at least two hydroxylating enzymes with distinct regioselectivity are involved. Comparison to free-cell fermentations in small- and large-scale bioreactors (up to 2000 ml) showed that the use of immobilized cells is advantageous, as they are easier to handle and yield higher amounts of oxidation products.