UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells

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• 作者：Wei Li ; Wu Di ; Lijuan Hua ; Bingrong Zhou ; Ze Guo ; Dan Luo ; ^{daniluo2005@yahoo.com.cn}
• 关键词：MicroRNA-141 ; PTEN ; UVB ; apoptosis ; HaCaT cells
• 刊名：Journal of Biomedical Research
• 出版年：2011
• 出版时间：March 2011
• 年：2011
• 卷：25
• 期：2
• 页码：135-140
• 全文大小：334 K

文摘

MicroRNAs (miRNAs) are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B (UVB) radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in HaCaT cells through an unknown mechanism. In this study, we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression. Real-time RT-PCR, annexin V/fluorescein isothiocyanate staining, Western blotting and anti-miRNA oligonucleotide transfection were employed in this study. We found that upregulation of miR-141 expression after UVB irradiation was inversely correlated with PTEN expression levels in HaCaT cells. Furthermore, miR-141 expression increased apoptosis, while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis effect of UVB. UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells. Therefore, miR-141 is a potential gene therapy target for UVB-induced photodamage.