Steroidogenesis and early response gene expression in MA-10 Leydig tumor cells following heterologous receptor down-regulation and cellular desensitization
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- 作者：Tsuey-Ming Chena ; Frank S. Czerwiecb ; David Puettc ; puett@uga.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：AP-1 ; activator protein 1 ; BSA ; bovine serum albumin ; CREB ; cAMP response element binding protein ; db-cAMP ; dibutryl cAMP ; EGF ; epidermal growth factor ; EGFR ; epidermal growth factor receptor ; ERG ; early response gene ; ERK1/2 ; extracellular signal regulated kinases ; EPAC ; exchange protein directly activated by cAMP ; hCG ; human chorionic gonadotropin ; IBMX ; isobutylmethylxanthine ; IP3 ; 1 ; 4 ; 5-inositol trisphosphate ; LHR ; luteinizing hormone receptor (also binds hCG) ; 4α-PE ; 4α-phorbol 12 ; 13-dideca
- 刊名：Biochemistry and Biophysics Reports
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：5
- 期：Complete
- 页码：305-312
- 全文大小：896 K
文摘
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Leydig tumor cells respond to hCG, cAMP, EGF, and phorbol esters with increased steroidogenesis.
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These same agents increase expression of the early response genes JUNB and c-FOS.
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Down-regulation of EGF receptors reduced hCG receptors and steroidogenesis.
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Desensitization of the PKC pathway reduced hCG receptors and steroidogenesis.
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Often expression of JUNB and c-FOS paralleled receptor loss, but not always.