The role of the 3鈥?region of mammalian gonadotropin 尾 subunit gene in the luteinizing hormone to chorionic gonadotropin evolution
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- 作者：Reut Gabay ; Shelly Rozen ; Albena Samokovlisky ; Yehudit Amor ; Rakefet Rosenfeld ; Fortune Kohen ; Abraham Amsterdam ; Peter Berger ; David Ben-Menahem
- 关键词：LH ; lutropin ; hCG ; human chorionic gonadotropin ; CTP ; carboxyl-terminal peptide ; wt ; wild-type ; CHO ; Chinese hamster ovary ; h ; human ; bo ; bovine ; AS ; antiserum ; BSA ; bovine serum albumin ; PBS ; phosphate buffered saline
- 刊名：Molecular and Cellular Endocrinology
- 出版年：15 February, 2014
- 年：2014
- 卷：382
- 期：2
- 页码：781-790
- 全文大小：1449 K
文摘
CG尾 subunits comprise a unique carboxyl-terminal peptide (CTP) that has multiple O-linked glycans and extends serum half-life of the protein. It has evolved by incorporating a previously untranslated region of the LH尾 gene into the reading frame. Although CTP-like sequences are encrypted in the LH尾 genes of several mammals, the CG尾 subunit developed only in primates and equids. To study this restriction in evolution, we examined whether the cryptic CTP decoded from the bovine LH尾 gene (boCTP) possesses key characteristics of the human (h) CG尾-CTP. The boCTP does not impede several crucial aspects of hormone biosynthesis, but compared to the hCG尾-CTP, the stretch lacks O-glycans and determinants for circulatory survival. O-glycan deficiency and the associated incapacity to extend serum half-life is a major drawback of the boCTP. This may explain why LH did not evolve into CG in ruminants and consequently alternative mechanisms evolved to delay luteolysis early in gestation.