Cardiac stem cell transplantation with 2,3,5,4′-tetrahydroxystilbehe-2-O-β-d-glucoside improves cardiac function in rat myocardial infarction model

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• 作者：Fan Song^a ; Fei Hua^a ; Hua Li^a ; Xuanxuan Zhou^a ; ^b ; Li Yan^a ; Qian Yang^a ; ^b ; Yanhua Xie^a ; ^b ; Weixun Duan^c ; Siwang Wang^a ; ^b ; ^{wangsiw@fmmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Jiyuan Sun^a ; ^b ; ^{sunjiy@fmmu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Cardiac stem cells ; Stem cell transplantation ; 2 ; 3 ; 5 ; 4&prime ; -tetrahydroxystilbene-2-O-β-d-glucoside ; Myocardial infarction ; C-kit ; Sca-1
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：1 August 2016
• 年：2016
• 卷：158
• 期：Complete
• 页码：37-45
• 全文大小：1900 K

文摘

Cardiac stem cells (CSCs)-transplanted therapy provides a promising therapy for the ischemic heart disease (IHD), especially in the epidemic of myocardial infarction (MI). The compound 2,3,5,4′-tetrahydroxystilbene-2-O-β-d-glucoside (THSG) can induce CSC proliferation in vitro based on our previous study, so we aimed to study the induce effect of THSG on CSCs-transplanted MI rat in vivo.

Materials and methods

Using a murine model of MI, this study was designed to evaluate the impact of THSG (30, 60, 120 mg/kg) on CSCs-based therapy for MI and the underlying mechanism in this process.

Key finding

The results showed that THSG on CSCs-transplanted therapy groups (THSG + CSCs groups) can significantly reduce S-T segment elevation, and increase heart rate compared with MI group. The left ventricular ejection fraction (LVEF) and the left ventricular fractional shortening (LVFS) were significantly reduced in THSG + CSCs groups compared to the MI group. The levels of enzyme expression (CK-MB, LDH), the heart weight index (HWI) and myocardial infarct size (IS) were all reduced in THSG + CSCs groups. Moreover, other changes noted during these 28 days post-MI, included pathologic changes, as well as increased stem cell antigen-1 (Sca-1) expression, or expression of Nkx2.5, GATA-4, and Connexin 43 in myocardial tissue, and reduced the Caspase-3 expression.

Significance

Our findings indicated that THSG facilitated CSCs-transplanted therapy in MI. These observations may be associated with the inducted of THSG on the proliferation of CSCs in vivo and also, with the subsequent differentiation of additional intrinsic neonatal cardiomyocytes to replace damaged heart tissue.