Inclusion of calcium during isolation of high-density lipoprotein from plasma maintains antioxidant function

详细信息    查看全文

• 作者：Sean M. Lynch ; ^{slynch@midwestern.edu" class="auth_mail} ; Joseph Lorenz ; Steven Klotz
• 关键词：Antioxidant ; Calcium ; High-density lipoprotein ; Low-density lipoprotein ; Oxidation ; Paraoxonase 1
• 刊名：Analytical Biochemistry
• 出版年：1 June 2014
• 年：2014
• 卷：454
• 期：Complete
• 页码：41-43
• 全文大小：360 K

文摘

We investigated how inclusion of calcium during isolation of high-density lipoprotein (HDL) affected its antioxidant function. Following isolation, HDL was dialyzed against 0.154 M NaCl without or with added calcium (1 mM). HDL’s paraoxonase 1 activity was unaffected by calcium treatment (87 ± 11% of normal vs. 89 ± 16% of normal, P = 0.826). In contrast, whereas HDL dialyzed with calcium inhibited oxidation of low-density lipoprotein (LDL) by 87 ± 10%, HDL dialyzed without calcium inhibited oxidation by only 58 ± 19% (P = 0.004). Thus, inclusion of calcium during isolation is important for maintaining HDL’s antioxidant function.