文摘
Semaphorin 4D (Sema4D)-treated dental pulp stem cells displayed marked down-regulation in the expression of osteo-/odontogenic genes (ALP, Col1A1, BSP, RUNX2, and DSPP) as well as proteins (Col1A1 and DSPP). Sema4D-treated dental pulp stem cells exhibited enhanced expression of vascular endothelial growth factor at both the messenger RNA and protein level. Sema4D markedly enhances the angiogenic potential but suppresses osteo-/odontogenic differentiation of dental pulp stem cells via the RhoA-mediated pathway. Elevated levels of plexin-B1 and downstream RhoA protein expression together with phosphorylated plexin-B1 confirmed the involvement of Sema4D/plexin-B1 signaling.