pSer40 tyrosine hydroxylase immunohistochemistry identifies the anatomical location of C1 neurons in rat RVLM that are activated by hypotension
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- 作者：P.E. Nedoboya ; S. Mohammeda ; K. Kapoora ; b ; A.M. Bhandarea ; b ; M.M.J. Farnhama ; P.M. Pilowskya ; c ; paul.pilowsky@hri.org.au" class="auth_mail" title="E-mail the corresponding author
- 关键词：BP ; blood pressure ; DMEM ; Dulbecco&rsquo ; s modified Eagle&rsquo ; s medium ; ELISA ; enzyme-linked immunosorbent assay ; FBS ; fetal bovine serum ; Fos ; protein product of the feline osteosarcoma oncogene &lsquo ; c-fos&rsquo ; IgG ; immunoglobulin G ; IHC ; immunohistochemistry ; mAbs ; monoclonal antibodies ; MAP ; mean arterial pressure ; MATF ; Monash Antibody Technology Facility ; PBS ; phosphate-buffered saline ; PFA ; paraformaldehyde in 0.1 ; M phosphate buffer ; PNMT ; phenylethanolamine-N-methyltransferase
- 刊名：Neuroscience
- 出版年：2016
- 出版时间：11 March 2016
- 年：2016
- 卷：317
- 期：Complete
- 页码：162-172
- 全文大小：1897 K
文摘
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Two monoclonal antibodies (mAbs) were made: one to TH and one to phosphorylated TH.
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The mAbs were of different isotypes allowing dual fluorescence immunohistochemistry.
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At rest, C1 neurons have a modest level of pTH, located mostly in the lateral RVLM.
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Following hypotension, the majority of C1 neurons in the RVLM become pTH-positive.
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These mAbs will enable the study of catecholamine neurons through the body.