Single-Cell Multiomics: Multiple Measurements from Single Cells

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• 作者：Iain C. Macaulay¹ ; ^{Iain.Macaulay@earlham.ac.uk} ; Chris P. Ponting² ; ³ ; ^{Chris.Ponting@igmm.ed.ac.uk} ; Thierry Voet² ; ⁴ ; ^{thierry.voet@kuleuven.be} ; ^{Thierry.Voet@sanger.ac.uk}
• 关键词：epigenomics ; genomics ; multiomics ; proteomics ; single cell ; transcriptomics
• 刊名：Trends in Genetics
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：33
• 期：2
• 页码：155-168
• 全文大小：2179 K
• 卷排序：33

文摘

Single-cell sequencing provides information that is not confounded by genotypic or phenotypic heterogeneity of bulk samples. Sequencing of one molecular type (RNA, methylated DNA or open chromatin) in a single cell, furthermore, provides insights into the cell's phenotype and links to its genotype. Nevertheless, only by taking measurements of these phenotypes and genotypes from the same single cells can such inferences be made unambiguously. In this review, we survey the first experimental approaches that assay, in parallel, multiple molecular types from the same single cell, before considering the challenges and opportunities afforded by these and future technologies.