KlHIS4-modulated histidine auxotrophy in yeast and characterisation of a KlHIS4-lacZ-based cadmium and copper biosensor

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• 作者：M.A. Freire-Picos and M. Lamas-Maceiras
• 关键词：Cadmium ; Copper ; Biosensor ; Yeast
• 刊名：Enzyme and Microbial Technology
• 出版年：2006
• 出版时间：6 December 2006
• 年：2006
• 卷：40
• 期：1
• 页码：67-70
• 全文大小：604 K

文摘

Based on the effects of cadmium and copper on KlHIS4 transcription when expressed in either Kluyveromyces lactis or Saccharomyces cerevisiae, a yeast biosensor is presented. It consists of a lacZ fused to a KlHIS4 447 bp long promoter expressed in K. lactis, and as control, a second fusion with a shorter promoter not regulated by metals. This eukaryotic system can be used to detect cadmium and copper concentrations of 0.3–3 mM (or higher) within minutes using X-GAL filter assay and other variants of β-galactosidase assays developed in multititer wells.