Salivary detection of periodontopathic bacteria in Fanconi's anemia patients

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• 作者：Karine Lyko ; Carmem Bonfim ; Elaine Machado Benelli ; Cassius Carvalho Torres-Pereira ; Jos茅 Miguel Amen谩bar
• 关键词：Periodontal pathogens ; Saliva ; Fanconi's anemia ; PCR
• 刊名：Anaerobe
• 出版年：December, 2013
• 年：2013
• 卷：24
• 期：Complete
• 页码：32-35
• 全文大小：238 K

文摘

Fanconi's anemia (FA) is characterized by bone marrow failure and can lead to infections such as periodontal disease. The aim of this study was to compare the prevalence of four periodontopathic bacteria including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Fusobacterium nucleatum and Treponema denticola in saliva samples from children with and without FA. Paraffin-stimulated saliva samples were collected from 71 children and adolescents, aged 6-18 years old. The samples were divided in three groups: FA without HSCT (n聽=聽25), FA with HSCT (n聽=聽23) and Non-FA (n聽=聽24). The test bacteria were identified using a 16S rRNA-based PCR analysis. P.聽gingivalis was the most frequent species in all groups and T.聽denticola the less frequent. P.聽gingivalis was also common species in combinations in all groups. No statistically difference was observed between the groups for single bacteria, pair or triple combination. The combination of the four species was detected in one saliva sample of the FA without HSCT group and in five samples of the Non-FA group, with statistical difference between them (p聽<聽0.05, Fisher exact test). Systemic alterations found in FA subjects did not affect the salivary distribution of the four bacteria analyzed.