The MIA pathway: A tight bond between protein transport and oxidative folding in mitochondria

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• 作者：Diana Stojanovski^a ; Piotr Bragoszewski^b ; Agnieszka Chacinska^b ; ^{achacinska@iimcb.gov.pl}
• 关键词：ERV1 ; essential for respiration and vegetative growth ; MIA ; mitochondrial intermembrane space assembly ; PDI ; protein disulfide isomerase ; SAM ; sorting and assembly machinery ; TIM ; translocase of the inner membrane ; ¦¤¦× ; membrane potential ; TOM ; transloca
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular Cell Research
• 出版年：2012
• 出版时间：July, 2012
• 年：2012
• 卷：1823
• 期：7
• 页码：1142-1150
• 全文大小：824 K

文摘

Many newly synthesized proteins obtain disulfide bonds in the bacterial periplasm, the endoplasmic reticulum (ER) and the mitochondrial intermembrane space. The acquisition of disulfide bonds is critical for the folding, assembly and activity of these proteins. Spontaneous oxidation of thiol groups is inefficient in vivo, therefore cells have developed machineries that catalyse the oxidation of substrate proteins. The identification of the machinery that mediates this process in the intermembrane space of mitochondria, known as MIA (mitochondrial intermembrane space assembly), provided a unique mechanism of protein transport. The MIA machinery introduces disulfide bonds into incoming intermembrane space precursors and thus tightly couples the process of precursor translocation to precursor oxidation. We discuss our current understanding of the MIA pathway and the mechanisms that oversee thiol-exchange reactions in mitochondria.