“Classic” myelin basic proteins are expressed in lymphoid tissue macrophages
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- 作者：Liu ; Hong-biao ; MacKenzie-Graham ; Allan J. ; Palaszynski ; Karen ; Liva ; Stephanie ; Voskuhl ; Rhonda R.
- 关键词：Macrophages ; Experimental autoimmune encephalomyelitis ; Myelin basic protein
- 刊名：Journal of Neuroimmunology
- 出版年：2001
- 出版时间：May 1, 2001
- 年：2001
- 卷：116
- 期：1
- 页码：83-93
- 全文大小：373 K
文摘
“Classic” myelin basic proteins (MBPs) are demonstrated in lymph nodes of SJL mice by western blot and RT-PCR. Interestingly, expression of these “classic” MBPs was increased during the late relapsing phase of adoptive experimental autoimmune encephalomyelitis (EAE). When splenocytes from SJL mice were separated into macrophage versus B lymphocyte-enriched populations, intact MBP isoforms were demonstrated in the macrophage-enriched population while undetectable in the B lymphocyte-enriched population. RT-PCR demonstrated “classic” MBP transcripts in splenic macrophages, as well as in a macrophage cell line (RAW). The expression of “classic” MBPs in lymphoid tissue macrophages raises the possibility that MBP-specific T cells may be exposed to autoantigen outside the central nervous system (CNS).