Cytotoxicity mechanism of α-MMC in normal liver cells through LRP1 mediated endocytosis and JNK activation
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- 作者：Ling Wanga ; 1 ; Fubing Shena ; 1 ; shenfubing@163.com" class="auth_mail" title="E-mail the corresponding author ; Min Zhangb ; Qianchuan Hec ; Hui Zhaod ; Xiaoping Yue ; Shuxia Yangf ; Yang Liuc ; Nianhua Denga ; Juecun Zhenga ; Lixia Zhua ; Xiaolan Liua
- 关键词：α-MMC ; α-momorcharin ; α2-M ; α2-macroglobulin ; LRP-1 ; low-density lipoprotein receptor-related protein-1 ; siRNA ; small interference RNA ; TCS ; trichosanthin ; JNK ; Jun N-terminal kinase ; RIPs ; ribosome inactivating proteins ; FITC ; fluorescein isothiocyanate ; CLSM ; confocal laser scanning microscope ; MAPK ; mitogen-activated protein kinases ; WB ; western blot ; Akt/PKB ; protein kinase B ; Bad ; Bcl-2 antagonist of cell death ; Bcl-2 ; B-cell lymphoma-2 ; CCK-8 ; Cell Counting Kit-8 ; IF ; immunofluorenscence
- 刊名：Toxicology
- 出版年：2016
- 出版时间：16 May 2016
- 年：2016
- 卷：357-358
- 期：Complete
- 页码：33-43
- 全文大小：2804 K
文摘
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α-MMC is more toxic on liver cells than on other normal cells.
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α-MMC can bind to membrane receptor and being competitively inhibited by α2-M.
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α-MMC can be endocytocesed into liver cells via LRP1 receptor and caused cell apoptosis.
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α-MMC could activate the JNK apoptosis signal transduction pathway.
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LRP1 knockdown can strongly inhibit the endocytosis of α-MMC and its JNK pathway.