Longitudinal evaluation of immunohistochemical findings of milk aspiration: An experimental study using a murine model

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• 作者：Tomonori Nagai^a ; Miwako Aoyagi^a ; Eriko Ochiai^a ; Kentaro Sakai^a ; Kyoko Maruyama-Maebashi^a ; Kenji Fukui^a ; Kimiharu Iwadate ; ^a ; ^{iwadate@jikei.ac.jp"" rel=""nofollow}
• 关键词：Sudden infant death ; Milk aspiration ; Immunostaining
• 刊名：Forensic Science International
• 出版年：2011
• 出版时间：15 June 2011
• 年：2011
• 卷：209
• 期：1-3
• 页码：183-185
• 全文大小：793 K

文摘

To examine the longitudinal change of pathological findings of the lung and other organs in milk aspiration, an experimental study using a murine model was carried out. Either 0.5 or 1.0 ml cow's milk was instilled into the trachea of rats. From immediately after to 14 days after instillation, the animals were sacrificed, and the lungs, liver, kidneys, and spleen were removed. The results of immunostaining with anti-human α lactalbumin antibody indicated that not only the lung but also the kidney and spleen showed a positive reaction against the antibody over time. Experimentally aspirated milk was detectable in alveoli until 2 days after instillation. It was also detectable in renal tubules from 1 to 6 h after instillation. Macrophages containing granules of aspirated milk were observed in splenic red pulp from 3 h to 14 days after instillation. Detection of aspirated milk in other organs except the lung would be clear evidence of intravital milk aspiration and would suggest previous or recurrent milk aspiration.