SPION primes THP1 derived M2 macrophages towards M1-like macrophages

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• 作者：Amit Laskar ; Jonas Eilertsen ; Wei Li ; Xi-Ming Yuan
• 关键词：Cathepsin L ; Ferritin ; Iron-oxide nanoparticles ; M1 and M2 macrophages ; Oxysterols
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：29 November, 2013
• 年：2013
• 卷：441
• 期：4
• 页码：737-742
• 全文大小：573 K

文摘

Potentially, cellular iron regulates functional plasticity in macrophages yet; interaction of functionally polarized macrophages with iron-oxide nanoparticles has never been studied. We found that monocyte differentiation alters cellular ferritin and cathepsin L levels and induces functional polarization in macrophages. Iron in super paramagnetic iron-oxide nanoparticle (SPION) induces a phenotypic shift in THP1 derived M2 macrophages towards a high CD86+ and high TNF 伪+ macrophage subtype. This phenotypic shift was accompanied by up-regulated intracellular levels of ferritin and cathepsin L in M2 macrophages, which is a characteristic hallmark of M1 macrophages. Atherogenic oxysterols reduce phagocytic activity in macrophage subtypes, and thus these cells may escape detection by iron-oxide nanoparticles (INPs) in-vivo.