The immunohistochemical overexpression of ribonucleotide reductase regulatory subunit M1 (RRM1) protein is a predictor of shorter survival to gemcitabine-based chemotherapy in advanced non-small cell lung cancer (NSCLC)
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- 作者：Jae Jin Lee ; Chi Hoon Maeng ; Seon Kyung Baek ; Gou Young Kim ; Jee-Hong Yoo ; Cheon Woong Choi ; Yee Hyung Kim ; Young-Tae Kwak ; Dae-Hyun Kim ; Young Kyung Lee ; Jung Bo Kim ; Si-Young Kim
- 关键词：Chemotherapy ; Gemcitabine ; Immunohistochemistry ; NSCLC ; RRM1
- 刊名：Lung Cancer
- 出版年：2010
- 出版时间：November 2010
- 年：2010
- 卷：70
- 期：2
- 页码：205-210
- 全文大小：301 K
文摘
We evaluated whether ribonucleotide reductase regulatory subunit M1 (RRM1) protein expression by immunohistochemistry (IHC) is a predictor of survival and response in gemcitabine-treated, advanced non-small cell lung cancer (NSCLC). We retrospectively collected 40 formalin-fixed, paraffin-embedded NSCLC tissues to investigate the protein expression of RRM1 by IHC with a purified rabbit anti-human RRM1 polyclonal antibody (ProteinTech Group, Chicago, IL, USA). RRM1 expression was positive in 14 (35 % ) and negative in 26 (65 % ) cases. Ten (25 % ) patients were treated as first-line and 30 (75 % ) patients as second-line. The median age was 61 years and M/F was 31/9. Stage IIIB/IV was 7/33 and adenocarcinoma/squamous cell carcinoma/other cell type was 20/16/4. Other characteristics, including age, gender, stage, cell type and first/second-line were not statistically different in the RRM-positive and RRM-negative groups. The overall survival of RRM1-positive groups was significantly shorter than RRM-negative groups (5.1 months vs. 12.9 months, p = 0.022). The response rates of 38 out of 40 patients were assessable. Disease control rate (PR + SD) of the RRM1-positive groups was significantly lower than that of RRM1-negative groups (23 % vs. 56 % , p = 0.053). In patients with gemcitabine-treated advanced NSCLC, patients with RRM1-positive tumors had worse overall survival and disease control than patients with RRM1-negative tumors.