- 作者：Julia L. Magnay ; M.Sc.a ; j.l.magnay@bemp.keele.ac.uk ; Gerrit Schö ; nicke ; M.D.b ; Tracy M. Nevatte ; Ph.D.a ; Shaughn O&rsquo ; Brien ; D.Sc.a ; Wolfgang Junge ; M.D.b
- 关键词：Heavy menstrual bleeding ; menstrual blood loss ; semiautomated alkaline hematin ; superabsorbent polymer granules ; ultrathin sanitary towels
- 刊名：Fertility and Sterility
- 出版年：2011
- 出版时间：August, 2011
- 年：2011
- 卷：96
- 期：2
- 页码：394-398
- 全文大小：260 K
Objective
To validate the semiautomated alkaline hematin technique for rapid measurement of menstrual blood loss on ultrathin sanitary towels with a superabsorbent polymer component.Design
Laboratory study using simulated menstrual fluid (SMF) and Always Ultra Normal, Long, and Night ¡°with wings¡± sanitary towels.
Setting
Laboratorium f¨¹r Klinische Forschung, Germany.
Patient(s)
None.
Intervention(s)
None.
Main Outcome Measure(s)
Linearity and blood recovery over a range of SMF volumes applied to towels, the lower limit of reliable detection, and the effect of storing soiled towels for up to 5 weeks at 20¡ãC and 4¡ãC before analysis, were determined. Recovery from 63 SMF samples comprising between 5 % to 100 % blood and 0.05-35 mL applied volume was compared with duplicates analyzed at Keele Menstrual Disorders Laboratory (manual reference method).
Result(s)
Linearity was confirmed, and ¡Ý85 % recovery was reproducibly achieved at up to 30 mL applied blood at all tested SMF compositions, except at low volume or high dilution equivalent to <4 mL blood. Samples could be stored for 3 weeks at 4¡ãC without loss of recovery. Linear regression analysis showed good agreement with the reference method.
Conclusion(s)
The semiautomated alkaline hematin technique is a reliable method for measuring menstrual blood loss from Always Ultra sanitary towels containing superabsorbent polymers.