Oleuropein aglycone counteracts A尾42 toxicity in the rat brain

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• 作者：Ilaria Luccarini ; Teresa Ed Dami ; Cristina Grossi ; Stefania Rigacci ; Massimo Stefani ; Fiorella Casamenti
• 关键词：AD ; Alzheimer's disease ; ChAT ; choline acetyltransferase ; OLE ; oleuropein aglycone ; NBM ; nucleus basalis magnocellularis ; A尾 ; amyloid 尾 ; NFT ; neurofibrillary tangles ; GFAP ; glial fibrillary acid protein ; Iba 1 ; ionized calcium binding adaptor molecule ; Ctx ; cortex ; LV ; lateral ventricle ; Str ; striatum.
• 刊名：Neuroscience Letters
• 出版年：13 January, 2014
• 年：2014
• 卷：558
• 期：Complete
• 页码：67-72
• 全文大小：2477 K

文摘

Previous data have shown that oleuropein aglycone (OLE), the main secoiridoid phenol present in extra virgin olive oil, counteracts in vitro aggregation of the A尾42 peptide and protects cultured cells and model organisms against aggregates toxicity. In this study we investigated the relative tissue toxicity of A尾42 aggregated in vitro in the presence or in the absence of OLE by injecting the nucleus basalis magnocellularis (NBM) of adult male Wistar rats with a 1.5 渭l solution containing OLE (450 渭M) or A尾42 (50 渭M) aggregated in the absence (oligomers) or in the presence of 450 渭M OLE. Control rats were injected with vehicle (1.5 渭l). Thirty days after injection, the number of choline acetyltransferase (ChAT)-positive neurons, glia reaction and the A尾 peptide levels were detected by immunohistochemistry. An apparent reduction in the amount of soluble A11-positive oligomers was detected in the NBM injected with A尾42 aggregated with OLE, as compared with the NBM injected with A尾42 alone. In the latter case, the number of ChAT-positive neurons was significantly reduced (鈮堚垝33%) respect to that recorded in the NBM injected with phosphate buffer, OLE or A尾42 aggregated with OLE. A markedly attenuated A尾-induced astrocytes and microglia reaction was also found in the NBM injected with A尾42 aggregated with OLE. Altogether, these data provide additional support to the anti-aggregation, neuroprotective and anti-inflammatory activities of this natural phenol, confirming its beneficial properties against neurodegeneration.