Influence of chondroitinase on indirect hemagglutination titers and phagocytosis of Pasteurella multocida serogroups A, D and F

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• 作者：Rimler ; Richard B. ; Register ; Karen B. ; Magyar ; Tibor ; Ackermann ; Mark R.
• 关键词：Pasteurella multocida ; Serology ; Phagocytosis ; Capsule ; Chondroitinase
• 刊名：Veterinary Microbiology
• 出版年：1995
• 出版时间：December, 1995
• 年：1995
• 卷：47
• 期：3-4
• 页码：287-294
• 全文大小：764 K

文摘

Capsules of Pasteurella multocida serogroups A, D and F contain mucopolysaccharides which block antigenic determinants and prevent phagocytosis. In this study, capsules of serogroup A, D and F strains of P. multocida were depolymerized by enzyme treatment. Capsule depolymerization of serogroup D and F strains with chondroitinase increased indirect hemagglutination (IHA) test titers and enhanced phagocytosis by swine neutrophils. Capsule depolymerization of serogroup A strains with hyaluronidase increased IHA titers, but depolymerization with chondroitinase did not. When serogroup A strains were treated with a combination of chondroitinase and hyaluronidase, IHA test titers were lower than titers of the same strains treated with hyaluronidase alone. Combined enzyme treatment of serogroup D strains resulted in IHA test titers similar to those of chondroitinase treatment alone.