Interaction of silica with cellulase and minimization of its inhibitory effect on cellulose hydrolysis

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• 作者：Md. Mahabubur Rahman Talukder ; ^{talukder@ices.a-star.edu.sg} ; ^{talukderbuet89@gmail.com} ; Hai Yuan Goh ; Sze Min Puah
• 关键词：Cellulase-silica interactions ; Adsorption isotherm ; Cellulose hydrolysis ; Inhibition
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：118
• 期：Complete
• 页码：91-96
• 全文大小：1006 K
• 卷排序：118

文摘

Silica was found to nonproductively adsorb cellulase during cellulose hydrolysis. The adsorption of cellulase on silica followed Langmuir isotherm and was endothermic. The adsorption study suggested strong interaction of silica with cellulase enzyme. Polyethylene glycol (PEG) improved cellulose hydrolysis yield 2.4-fold. PEG did not only reduce cellulase adsorption on silica but also enhance its activity.