In vitro human CD4+ T cell response to the vaccinia protective antigens B5R and A33R

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• 作者：Philemon Sirven ; Florence Anne Castelli ; Alicia Probst ; Natacha Szely ; Bernard Maillere
• 关键词：HLA class II ; Epitopes ; CD4+ T helper lymphocytes ; Poxvirus
• 刊名：Molecular Immunology
• 出版年：2009
• 出版时间：April 2009
• 年：2009
• 卷：46
• 期：7
• 页码：1481-1487
• 全文大小：754 K

文摘

Subunit vaccine candidates against poxvirus infection induced protective humoral and cellular response in animal models but their immunogenicity in human remains unknown. We have therefore evaluated in vitro the CD4 T cell response of the major antigens B5R and A33R and characterized their CD4 T cell epitopes. Twelve peptides selected on the basis of their binding capacity to HLA-DR molecules, induced CD4 T lymphocytes harvested in healthy donors. In the A33R proteins two peptides are T cell stimulating for at least half of the donors and are restricted to multiple HLA-DR molecules in agreement with their broad specificity for HLA-DR molecules. In B5R, two peptides exhibited a good immunoprevalence but only one is a good binder to multiple HLA-DR molecules. One peptide was a moderate binder for multiple HLA-DR molecules, although it was efficiently presented to peptide-specific T cell lines. Altogether, our data demonstrated the capacity of B5R and A33R peptides to elicit a T cell response in multiple healthy donors and showed that promiscuity and immunoprevalence of CD4 T cell epitopes are not necessarily associated.